%A Wang Jianxun*, Zeng Tian, Zhang Peng, Wang Zhigang, Lei Hailu. %T Effects of tumor suppressor NDRG2 gene on proliferation and invasive ability of colon cancer cell line SW620 %0 Journal Article %D 2016 %J Journal of International Oncology %R 10.3760/cma.j.issn.1673-422X.2016.06.001 %P 401-404 %V 43 %N 6 %U {https://gjzlx.sdfmu.edu.cn/CN/abstract/article_9968.shtml} %8 2016-06-08 %X Objective To observe the influence of N-myc downstreamregulated gene 2 (NDRG2) on the growth and invasive ability of human colon cancer cell line SW620, and to explore its mechanism. MethodspcDNA3.1NDRG2 and siRNANDRG2 were transfected transiently respectively into SW620 by Lipofectamine TM 2000, untreated cells as the control group. Western blotting was used to investigate the expression of NDRG2 and matrix metalloproteinase-2(MMP-2). Matrigel invasion assay was used to study the invasive abilities of SW620 cells in all groups. The growth curve was determined through 3(4,5dimethyl2thiazoly)2,5diphenyl2Htetrazolium bromide method. ResultAfter transfecting pcDNA3.1NDRG2 into the SW620 cells, the protein level of NDRG2 increased and the expression of MMP-2 declined markedly. After transfecting siRNANDRG2 into the SW620 cells, the protein level of NDRG2 declined and the expression of MMP2 increased markedly. In addition, compared with the control group (75.80 ±4.82), the numbers of transmembrane cells in pcDNA3.1 group (56.20 ±7.40) and in siRNA group (94.20 ±9.23) were significantly different (t=13.102, P=0.000; t=11.820, P=0.000). The growth curve showed that: compared with the control group (0.67±0.01), the absorbance of the fifth day after transfection in pcDNA3.1 group (0.46±0.01) and in siRNA group (0.91±0.02) were different significantly (t=9.561, P=0.000; t=10.922, P=0.000). ConclusionNDRG2 can reduce the invasion and proliferation ability of colon cancer cell SW620, and its mechanism may be related to the downregulation of MMP-2 expression.