%A Liu Wenbin, Gao Nina %T Clinical significance of miR-210 expression in breast cancer tissues and its influence on malignant behavior of triple negative breast cancer cells %0 Journal Article %D 2018 %J Journal of International Oncology %R 10.3760/cma.j.issn.1673-422X.2018.09.003 %P 525-530 %V 45 %N 8 %U {https://gjzlx.sdfmu.edu.cn/CN/abstract/article_10510.shtml} %8 2018-09-08 %X ObjectiveTo investigate the expression and clinical significance of microRNA210 (miR210) in breast cancer tissues, and to investigate its effect on the proliferation and metastasis of human triple negative breast cancer cell line MDAMB231 in vitro. MethodsThe breast cancer tissues and paracancerous tissues in 82 patients were collected in the Department of Pathology of Hunan Cancer Hospital from December 2013 to September 2015. Quantitative realtime polymerase chain reaction (qRTPCR) technique was used to detect the expression level of miR210 in tissues and cells. The relationship between the expression of miR210 and clinical data and prognosis of patients were analyzed. The triple negative breast cancer cell line MDAMB231 transfected with fulllength miR210 plasmid was regarded as test group, and the cell transfected with blank vector was regarded as control group. CCK8 assay was used to detect the proliferation ability of cells in both groups. Transwell invasion and migration assays were used to detect the metastasis and invasion ability of cells. ResultsThe results of qRTPCR showed that the expression level of miR210 was 0.198±0.014 in breast cancer tissues, which was significantly higher than that in paracancerous tissues (0.084±0.009), and the difference was statistically significant (t=8.141, P＜0.001). The expression level of miR210 in triple negative breast cancer tissues was 0.254±0.026, which was significantly higher than that in nontriple negative breast cancer tissues (0.167±0.015), and the difference was statistically significant (t=3.175, P=0.003). There were significant differences in TNM staging and molecular typing between the patients with high and low expression of miR210 (χ2=7.859, P=0.005; χ2=7.053, P=0.008). The 4year survival rate of patients with high expression of miR210 was significantly lower than that of patients with low expression of miR210 (49.37% vs. 76.80%), and the difference was statistically significant (χ2=4.743, P=0.024). The results of qRTPCR showed that the expression of miR210 in cells in test group was 0.517±0.038, which was significantly higher than that in control group (0.284±0.022), and the difference was statistically significant (t=9.280, P＜0.001). The results of CCK8 assay showed that the proliferation abilities of the test group were significantly higher than those of the control group in 48, 72 and 96 h (3.771±0.452 vs. 3.206±0.314; 7.662±0.619 vs. 6.736±0.552; 15.477±1.425 vs. 11.592±1.243), and the differences were statistically significant (t=2.296, P=0.025; t=2.496, P=0.019; t=4.594, P=0.001). The results of Transwell invasion assay showed that the cell number of test group in inferior surface was 107.8±13.0, which was significantly higher than that of control group (74.4±10.9), and the difference was statistically significant (t=3.732, P=0.001). The results of Transwell migration assay showed that the cell number of test group in inferior surface was 136.5±18.5, which was significantly higher than that of control group (87.4±15.7), and the difference was statistically significant (t=4.256, P＜0.001). ConclusionThe expression of miR210 in breast cancer tissues is high, and its expression is closely related to progression, malignancy and prognosis of patients. In vitro, miR210 can promote the malignant behavior of triple negative breast cancer cell line MDAMB231. It is a potential molecular marker and targeted treatment site.