%A Pan Yukai
%T Expression and clinical significance of microRNA-148a in patients with non-small cell lung cancer
%0 Journal Article
%D 2016
%J Journal of International Oncology
%R 10.3760/cma.j.issn.1673-422X.2016.08.003
%P 570-573
%V 43
%N 8
%U {https://gjzlx.sdfmu.edu.cn/CN/abstract/article_10016.shtml}
%8 2016-08-08
%X Objective&nbsp; To investigate the expressions of microRNA-148a (miR-148a) in non-small cell lung cancer (NSCLC) tissues and adjacent non-tumor tissues and the relationships with clinicopathologic features, to analyze the roles of miR-148a in evaluating prognosis. MethodsExpression levels of miR-148a in 48 pairs of NSCLC and adjacent non-tumor tissues were detected by quantitative real-time PCR (RT-PCR). The patients were divided into 2 groups according to the median value of miR-148a. The relationships between the expression levels of miR148a and clinicopathological features were analyzed. The survival rate was estimated by KaplanMeier method, and single factor analysis was performed. The relationship between the expression level of miR-148a and prognosis was analyzed by Logrank test. Results&nbsp; miR-148a expression levels were significant down-regulated in NSCLC tissues compared with adjacent non-tumor tissues (1.052 &plusmn; 0.659 <em>vs</em>. 1.397 &plusmn; 0.667, <em>t</em>=2.549, <em>P</em>=0.013). miR-148a expression level was correlated with tumor size (&chi;<sup>2</sup>=4.594, <em>P</em>=0.030), lymph nodes metastasis (&chi;<sup>2</sup>=5.486, <em>P</em>=0.019) and clinical stage (&chi;<sup>2</sup>=4.090, <em>P</em>=0.043), while it was not correlated with age (&chi;<sup>2</sup>=0.354, <em>P</em>=0.551), gender (&chi;<sup>2</sup>=2.277, <em>P=</em>0.131), histological type (&chi;<sup>2</sup>=0.403, <em>P</em>=0.525) and smoking (&chi;<sup>2</sup>=0.444, P=0.505). KaplanMeier analysis revealed a significant correlation between low miR148a expression level and poor overall survival (23.9 months vs. 38.7 months, &chi;2=4.941, P=0.026), and no significant correlation with progressionfree survival (21.6 months vs. 29.4 months, &chi;<sup>2</sup>=2.168, <em>P</em>=0.141). Conclusion&nbsp; Down-regulation of miR-148a is correlated with worse clinicopathological features, which serves an independent marker for poor prognosis in patients with NSCLC.